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Plasmid dilutions were generated to span the anticipated transcript copy range (10 10 copies).
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Absence of GATA-tagged transcripts in lung/heart is anticipated to be their transcriptional quiescence whereas tissue-specific transcripts entailed their exclusive requirement in the respective tissues.
Consequently we anticipated that our transcriptional analyses could identify transcripts present in both heart fields, as well as those exclusively present in one heart field.
In particular, we provided evidence that the coding potential of the T. brucei genome was larger than originally anticipated by identifying 1,114 transcripts mapping to regions of the genome with no annotated ORFs [ 28].
As anticipated from this small overlap, transcript and protein abundance was only weakly related to each other when all detected mRNAs and proteins were considered (R = 0.19; Fig. 4a).
In summary, these data verified the array signal was indeed dependent on the transcript concentration, as anticipated.
It may have been anticipated that often such nested transcript annotations would simply reflect artefactual ESTs arising from truncated first strand cDNA synthesis with no biological relevance.
We anticipated that this pathway-based transcript panel would report major variances in gene expression programs across very diverse cell-types.
Since C. merolae has only one mitochondrion and one chloroplast, we anticipated that changes in organelle transcript levels in response to external and internal conditions may be more easily recognizable in this organism.
As anticipated, except for galR, no transcripts of the 12 deleted contiguous genes were detected.
All primer sets yielded unique dissociation curves indicating the presence of Rnf14 transcripts in the anticipated samples.
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