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Here, we would like to comment on the anticipated specificity of these kinases, which in the past have been assumed due to their primary sequence and selected biochemical analyses to specifically phosphorylate serine and threonine residues [for review 8].
To facilitate the use of these modules for diverse purposes, we here present extensive biophysical characterization of numerous SYNZIP interactions in vitro and report the ability of many pairs to interact with the anticipated specificity in yeast.
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anticipated, greater specificity in differentiating relationship qualities than did field-dependent clients.
In one Boston-based ED experience, patients with a reactive oral Oraquick HIV test had an 8- to 32-fold increased odds of HIV infection compared with the pre-test odds, despite a lower than anticipated test specificity [23].
Investigators have noted that it is realistic to anticipate that specificity would improve with additional training and experience to the equivalent of radiologists reading screening mammograms.
It is anticipated that the ion-specificity of at least some antiporters will parallel that of the major coupling ions for the voltage-gated channels that provide a significant amount of their cytoplasmic substrate.
It is anticipated that the position specific information on substrate specificity, structural (of the enzyme and substrate) and localization information will increase the accuracy of the prediction methods and eliminate the false positive ones from the ensemble.
There were fewer false-positive MRI scans that had been anticipated because of the better specificity of MRI than had been predicted at the outset of the study.
Given the uncertain proton configuration and the flexibility of both ligand and protein, we did not anticipate finding the exact binding orientation of 4-MDDT in the active site but anticipated some insights into ligand specificity.
We attempted to raise an antibody, but as the reviewers have anticipated, we could not establish the specificity of the antibody generated.
These cell culture-based observations highlight a more complex relationship between histone H3K4me demethylation and transcription regulation than was initially anticipated and suggests that methylation state specificity could underpin the differing activity of H3K4 demethylases at gene promoters and enhancers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com