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As anticipated, mutations in the major murein hydrolase, atlA, possessed low levels of autolytic activity.
The data revealed an excess of mechanistically anticipated mutations in subjects treated with the drug, suggesting that VDA is applicable to human treatment.
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DOI: http://dx.doi.org/10.7554/eLife.04126.018 In addition to affecting kinase selectivity at the +1 position, as anticipated mutation of the DFGx residue also impacted phosphoacceptor preference (see Figure 4, Figure 4 figure supplement 2).
"Like beads on a necklace, we can shift from one to the next in order to anticipate mutations".
While our goal has been to identify genes that are important for membrane dynamics in the adult gonad during oogenesis, we anticipated that mutations in genes required more generally for metabolism and gene expression, without being directly involved in the regulation of membrane dynamics, might also cause both eggshell defects and adult sterility after L1 and L4 up-shifts, respectively.
However, with the rapid advances made by "next generation sequencing", it is anticipated that mutations will soon be identified for almost all patients with genetic disorders; the International Rare Disease Research Consortium (IRDiRC) has set itself an ambitious goal of having the means available to diagnose most rare genetic diseases by 2020 (http://www.irdirc.org).
Therefore, we anticipated that mutation or phosphorylation of Y1065 within Vt would not affect PIP2 association.
Good doctors, the Classic of the Yellow Emperor advises, will anticipate the mutations of these energies before they become catastrophic and facilitate a new balance of the qi.
This strategy requires anticipating beneficial mutations and having a way of identifying them in the population once they arise, quickly and efficiently.
In addition to identifying HSR thermostat mutants we also anticipate discovering mutations which disrupt the wave like expression dynamics of Hsp17.6p GFP.
If we succeeded at all in getting activity enhancing mutations we anticipated obtaining two types of mutations.
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