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Although we anticipated gene expression signatures that were unique to dNFSCs or pNFSCs, transcripts that passed the ANOVA failed to partition the two NF subtypes.
As the anticipated gene expression effects that might be expected in crebKO tissue were not observed, we isolated SI crypts for gene expression studies (these were depleted of the villi) from three creb/villinCre ERT2 and three creb fl/fl control mice where each cohort had been subjected to 4-week Tamoxifen treatment.
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As anticipated, gene ontology (GO) analysis indicated that most bHLH genes are related to the regulation of macromolecule metabolic processes and gene expression.
Furthermore, we have initiated a pineapple microarray project and it is anticipated that gene expression data will be incorporated into the online pineapple bioinformatics resource in the future.
As anticipated, altered gene expressions were noted in AML-2/IDAC cells, as compared with the AML-2 cells.
To be deleterious, changes such as inversions, insertions or copy number variations would be anticipated to change gene expression levels, and no changes in mRNA levels were detected.
This study illustrates a very tight concordance between both methods with a significant difference that NRO appears to anticipate changes in gene expression at time points earlier than they appear in total RNA, consistent with altered transcriptional rates followed by changes in total RNA.
As anticipated, part of this gene expression pattern is consistent with expression of known IFN response genes [13].
Furthermore, we anticipated that breed-related gene expression profiles would uncover biologically and therapeutically significant pathways that would inform etiology and identify therapeutic targets.
Protein expression levels of Fn1, Chga, Lrrn4, and Il11ra1 were elevated in prion-resistant R7 compared to susceptible S7 cells as anticipated from the corresponding gene expression data (Fig 2).
More specifically, we anticipated observing clustering of gene expression predominantly among the in planta conditions (rice and barley), the nutrient deprived conditions (MM-C and MM-N) and the reactive oxygen species condition (PQ).
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