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An affinity-purified, anti-PLCζ polyclonal antibody positively detected a single, immunoreactive 70 kd protein corresponding to human PLCζ (5), with the control anti-β-actin antibody identifying a 42 kd human β-actin band (Fig. 1A).
Previously, we have analyzed chromatin immunoprecipitation on tiling array (ChIP-on-Chip) using a TCF7L2 antibody identifying both the Isl1 and Ins1 promoters as directly bound by TCF7L2 (30).
Primary antibodies used were a rabbit monoclonal antibody to ATM (clone Y170, 1 1000, Abcam, Cambridge, MA, USA), a mouse monoclonal antibody to vimentin, which marks the stromal compartment (clone V9, 1 5000, DAKO), and a guinea pig monoclonal pan-cytokeratin antibody identifying the tumor compartment (1 100, Acris, San Diego, CA, USA).
Immunohistochemical methods showed that the carriers compared to the non-carriers had a higher percentage of type II fibres stained with the antibody identifying type IIA and IIX fibres in m. longissimus dorsi and a lower percentage of type IIB fibres in both m. biceps femoris and m. longissimus dorsi.
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Finally, immunodetection for cytochrome c, again with co-labeling using calbindin D28k antibody, identified intracellular release of cytochrome c from the mitochondria into the cytoplasm of Purkinje cells.
IDEC was developing the experimental drug, an antibody identified as IDEC-131, to treat Crohn's disease and multiple sclerosis, among other diseases.
For reference, the anti-PPMLC20 antibody identifies only the uppermost band in these last three panels, which corresponds to PPMLC20.
The dual-phosphorylated form of ERK (p-ERK) antibody identified two bands of approximately 44 and 42 kDa (corresponding to p-ERK1 and p-ERK2, respectively).
The anti-Flag tag antibody identifies one of the ends of the extended NECD as the position where the receptor inserts into the membrane (Figure 3B).
ICC using avian GnIH antibody identified a group of GnIH-ir neurons in the dorsomedial region of the human hypothalamus (Fig. 1A, 1B).
In contrast, a Muc5ac monoclonal antibody identified bands in the mucus samples from infected mice.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com