Exact(1)
If the anti-dsDNA antibody criterion in SLE classification is to remain useful, its definition should represent current trends and insights.
Similar(59)
Breast cancer samples classified as ER-positive according to both biochemical (cytosolic DCC assay) and histochemical (ER1D5 monoclonal antibody) criteria were labelled with [H]oestradiol and were subsequently subjected to hydroxylapatite adsorption.
[1] Of particular importance in context of the present study are criteria linked to development of kidney disease: Production of potentially pathogenic anti-dsDNA antibodies (criterion # 10) and deposition of chromatin-containing immune complexes in kidneys (criterion # 7).
This discrepancy might be due to the use of different antibodies, criteria of evaluation and other issues related to discordant study protocols [ 38] and, considering the potential importance of FMCs as model for human breast cancer, a standardised method for the detection of HER2 expression and cellular localisation in feline mammary tumours is urgently needed.
However, the optimal methods of detecting antibody and criteria for seropositivity to H7 virus in humans remain unclear; current criteria used are those established and adapted by the WHO for H5N1 subtype human infection and extrapolated for the H7 subtype.
The image analysis software automatically enumerates cells based on antibody selection criteria and reports a sensitive and quantitative measure of protein levels, termed "mean fluorescence intensity" (MFI).
Successful strategies for the development of monoclonal antibodies require integration of knowledge with respect to target antigen properties, antibody design criteria such as affinity, isotype selection, Fc domain engineering, PK/PD properties and antibody cross-reactivity across species from the early stages of antibody development.
Based on this antibody selection criteria (>75% OD of positive control), we estimate that 28% of B cell cultures (n = 1,800) derived from 21 patients, each arising from 500 B cells, produced antibodies that recognized metastatic melanoma cells, compared to 2% (n = 600) of cultures derived from 10 healthy volunteers (Figure 4, B).
All patients met at least clinical, EEG, and CSF antibody titer criteria defined by Dyken (8 ).
In selected sera, confirmatory assays using recombinant or synthetic antigens (see Table 1 Antibody detection criteria) were performed.
At least 2 and probably 3 LNds are CRY-negative by antibody staining criteria even after many days of extended incubation in DD (Yoshii et al., 2008).
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