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All three media are suited for capture of feed stocks with high antibody content.
One hundred and ninety-two sera had earlier been analysed for total HHV-6 antibody content in a competitive ELISA, 94 sera were analysed for cytomegalovirus (CMV) IgM and 57 sera for Epstein Barr virus (EBV) antibodies, using different ELISA assays.
The serum samples were also screened for the presence of anti-FMDV antibodies (using a 1∶10 dilution of serum) using serotype specific antibody ELISA systems (for O and Asia 1 as appropriate) and positive samples were then titrated to quantify the antibody content [15], [16].
Anti-H5N1 antibody content of serum samples was measured by ELISA as described previously [ 53].
Antibody content of these VWs served as a surrogate for mucosal immune responses.
The antibody content in the fodder and in feces was controlled by the BA11-specific ELISA.
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Sera samples were obtained by venous puncture at the start of the survey (in November 2000) from each child of the cohort and the parasite-specific antibody contents from this first year blood samplings were evaluated by ELISA.
We paid special attention to keeping the filter papers dry and found no significant decay of antibody contents.
After incubation with a horseradish-conjugated goat anti mouse antibody, the protein content was visualized using enhanced chemiluminescence (Amersham, Freiburg, Germany).
Incorporated BrdU was detected using monoclonal antibodies and DNA content was determined by propidium iodide staining (for details of the methods, see Thews et al, 1996).
The traditional assay used to measure potency of inactivated influenza vaccines is a single-radial immunodiffusion (SRID) assay that utilizes an influenza strain-specific antibody to measure the content of virus hemagglutinin (HA) in the vaccine in comparison to a homologous HA reference antigen.
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CEO of Professional Science Editing for Scientists @ prosciediting.com