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In addition, the functional comparison of antibody bound and naked GFP-D2 receptors revealed no differences in signaling.
One microgram of anti-Flag or anti-T7 antibody bound to 50 µl of 50% protein G-sepharose slurry was incubated with 500 mg of nuclear extract overnight.
The sandwich immunoassay consisted on an "antibody-antigen-labelled antibody" bound with extracted epitopes from 5 ml of treated and non-treated Yersinia cultures.
Standards and samples (~200 μL) were pipetted into the wells and the immobilized antibody bound any antigen of interest (IL-1 β, IL-6 or TNF-α) present.
This antibody bound CCK-8 and CCK-33 with nearly equal affinity.
Moreover, 5E9 antibody bound to variable numbers of Sezary, acute myelogenous leukemia, and ALL PB leukemia cells.
Similar(29)
Finally, antibody-bound protein complexes were eluted with 0.2 M glycine pH 2.0, eluates neutralized with 1 M Tris pH 9.0, and subjected to MS analysis.
To determine whether the antibody-bound receptors were functional, electrophysiological recordings were made from cells having antibody labeled D2 receptors (Supplementary Figure 3A).
Antibody-bound DNA were washed and treated with Proteinase K and RNase A and crosslinking was reversed by incubation at 55 °C for two and a half hours.
The indicator cells form agglutinates (rosettes) around the antibody-bound Rh(D -positive fetal cells if present.
Free and antibody-bound tracer were separated by Sac-Cel separation.
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