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(B) Plasmid extracted from transformants on LB+ antibiotic plate showed reappearance of plasmid band (The full length gel image is shown as Supplementary Fig. 7) and (C) the transformed strain exhibited elevated MIC for meropenem.
This is the antibiotic plate.
Antibiotic plate bioassay was performed as described previously [5].
The antibiotic plate was inoculated with bacteria from the overnight growth plate by stabbing the wells of the overnight growth plate with a sterile toothpick and then stabbing the antibiotic plate.
After 12 h of infection we isolated intracellular bacteria from the infected cells and the bacteria were plated in antibiotic plate to enumerate the number of bacteria present for the different strains.
Isolates were scored as "1" for growth (resistant) or "0" for no growth (susceptible) for each antibiotic plate.
Similar(53)
lux-PCR confirmed the stability of the insert in all the randomly selected colonies of A. baumannii strains from the antibiotic plates.
For the three-way outcrossing trials, the same procedure was used, but mating plates were replica plated to three single antibiotic plates, then to three double antibiotic plates.
Once colonies formed, the mating plates were replica plated to single antibiotic plates to verify the viability of at least one spore in each ascus and then to double antibiotic plates to ascertain outcrossing.
The bacteria were plated in antibiotic plates to enumerate the number of bacteria present for the different strains.
24 h later, 3 samples from each mating plate were plated on permissive medium and subsequently replica plated to double antibiotic plates to estimate outcrossing frequency.
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