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Plants in anthesis with similar growth trends were chosen for the experimental treatment.
Berries were collected at nine time points during the berry development season (spanning the period three to 19 weeks post anthesis), with 10 14 day intervals.
Wheat spikes from Wangshuibai and NAUH117 were point-inoculated at anthesis with freshly prepared spore suspension.
Previous reports in species with wet stigmas have shown a fairly well-conserved structure at anthesis with papillae covered with secretion and a stigmatoid tissue beneath (Konar and Linskens, 1966; Ciampolini et al., 1990; Heslop-Harrison, 2000).
Twenty plants for 02-5B-318 and Saragolineline were artificially spray-inoculated during anthesis with a 10/mL distilled-water macroconidia suspension, for each plant 5 spikes were chosen for a total of 100 spikes per lines.
The temporal and spatial cell division pattern during petal development was analysed by conducting in situ hybridisation studies in younger Iberis flower buds before anthesis with the cell cycle marker gene Histone4 from Iberis amara (IaH4).
Similar(50)
Unless specified otherwise, plants were spray inoculated at mid-anthesis with F. graminearum isolate CS3005 [ 50].
The relationship between fresh weight (FW), dry weight (DW), sugar concentration, and cell wall digestibility of the fourth internode and that of the whole stalk was determined using 15 mature, field grown sweet S. bicolor genotypes harvested at 10 days post-anthesis with a height and FW range of 237 to 338 cm and 228 to 941 g (Additional file 3), respectively.
In this experiment, two rice genotypes with different heat tolerance were subjected to heat stress at anthesis pretreated with NAA and the auxin synthesis inhibitor L-aminooxyphenylpropionic acid (AOPP) (Soeno et al. 2010) to evaluate the role of auxin in pollen tube growth in the pistil and spikelet fertility under heat stress, as well as its underlying mechanism.
Production of this secretion is already seen before flower opening and reaches a maximum level 1 d after anthesis, concomitant with pollen tube passage.
Pollinated flowers, on the other hand, maintain a very low level of ethylene biosynthesis and signalling in the fruit during the days immediately after anthesis, concomitantly with fruit set and development.
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