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RT-qPCR revealed that mRNAs of all three monoglutamate folate biosynthesis genes (Fig. 8a, b and c) were elevated at the anthesis stage in the fpgs03g line.
a Leaf rolling, b Leaf wilting, c Leaf yellowing, d Leaf dryness, e Spike damage (booting stage) and f shrunken seeds (anthesis stage).
In an informal survey, Fusarium head blight (FHB) was found to be prevalent in many counties after the anthesis stage of wheat (Kuo et al. 2014).
T is T mean for wheat and filling stage of maize, and T is T max for rice and anthesis stage of maize.
Also, drought stress during the anthesis stage drove the stressed plant to maturation faster, shortening the milk and dough stage resulting in shrunken seeds without generating new leaves.
Since embryo development occurs within a few days after anthesis stage (Counce et al. 2000), impaired folate polyglutamylation is likely to impact the rate of DNA synthesis, causing a delay in embryo development.
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We then used this array to analyze floral gene expression in late pre-anthesis stage floral organs from a natural A. formosa population.
Sixty flowers, all from late pre-anthesis stage, were harvested from each population, dissected into the five floral whorls (sepals, petals, stamens, staminodia, carpels) and immediately frozen into liquid nitrogen.
There were also five genes with higher expression at flower bud and 3DPA, and three with higher expression at the flower bud to pre-anthesis stage.
From each variety, tissue was sampled from the second and third true leaves, and from male and female flowers in pre and post-anthesis stage.
However, the presence of this specific "single-ovular" Ca2+ labelling was still observed at the post-anthesis stage of flower development (stage 5) when most of the flowers were successfully fertilized.
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