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At the maximal concentration tested, ANP (1 μM) increased basal cGMP content by 14 ± 3-fold in WT and by 8 ± 1.2-fold in Cav-1-deficient MLECs.
To quantify the urine ANP, 1 mL of urine was extracted using Sep-Pak C18 cartridges (Waters Corporation, Milford, MA, USA), as previously described [ 29].
Indeed Ropero and colleagues showed that ANP 1 nM dampened KATP activity in cell-attached patches from mouse pancreatic beta cells, illustrated by a 50%% reduction in NPo compared to no-treatment control [ 36].
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It has reported that another type of ANP, ANP 1 28, also has inhibitory effect on ICaL in rabbit heart cells [18].
To examine the co-localization of eGFP NPRA with early endosome marker EEA-1, cells were treated with 100 nM ANP for 5, 10, 15 and 30 min.
Cells were treated with 100 nM ANP for 5, 10, 15 or 30 min in the presence of 0.2 mM IBMX.
To visualize the eGFP-tagged receptor, cells were treated with 100 nM ANP for 5, 10, 15 and 30 min.
To examine if this type of ANP has the similar effect on ICaL as ANP 3 28 in our model, equal concentration of ANP1 28 was tested.
A novel 1D polymer, [Cd anp 2Br2]·H2O (1) (anp = 2-amino-5-nitropyridine), was synthesized through the solvothermal reaction of CdBr2·4H2O with anp.
Furthermore, increased ADM correlated positively with elevated creatinine (r=0.76, P=0.01) and ANP (r=0.64, P=0.04) after liver transplantation.
In HPLC analysis the majority of the pericardial fluid and tissue ir-ANP coeluted with human ANP [99 126].
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