New York held its leadership position for another three generations and provided nearly one million industrial jobs into the 1950s.
In each repeat, developmentally synchronized animals were first cultured at 15 °C for three generations (referred to as 15C-G1, 15C-G2, and 15C-G3, "G" for Generation) and then at 23 °C for six generations (23C-G1 to 23C-G6), followed by another three generations at 15 °C (p15C-G1, p15C-G2, and p15C-G3, "p" for p ost-heat stress) (Fig. 1a).
[Genbank: DQ251037] The mutation in another three generation family ANF8 was originally detected by SSCP in exon 5 (Fig. 4A, B) of the paired domain and was further analyzed by sequencing the PCR product of affected individuals and unaffected relatives from the family.
I hope that I won't have to wait another five generations.
This allowed an unchallenged Fidel Castro to remain in power for another two generations — outlasting nine American Presidents and even the Soviet Union — and by his staying power and unstinting audacity, to earn himself an enduring place in modern history.
Future discoveries may alter the math, but, as it now stands, forty-five hundred generationseparatethethearliestst Homo sapiens from the earliest cave artists, and between the artists and us another fifteen hundred generations have descended the birth canal, learned to walk upright, mastered speech and the use of tools, reached puberty, reproduced, and died.
"Otherwise, we're going to sit here for another two generations".
Older SaaS companies can work with former on-premise foes and happily proclaim that what worked for the past 14 years will be just fine for another two generations or more.
Even the young folks who sing Cajun tunes in local bands mostly don't speak the language... give them another two generations, and it'll be like church choirs that sing Latin.
We continued outcrossing for another four generations (10 generations in all), and used an outcrossed lamP oc) stock for subsequent experiments.
In contrast, in the PAI gene family, combining the master locus (WS ecotype PAI1/PAI4) with a non-allelic singlet target locus possessing 100% sequence identity (Columbia PAI2) resulted in methylation after two generations of heterozygous contact, and methylation became more pronounced after another two generations [ 12].
