Exact(9)
The sources of the remaining events could not be identified, and the anomalous sequence variation referred to matrilines from East Asia, Africa, or West Eurasia, respectively.
Using the original first PCR products of these cells as the template and re-performing the second PCR and sequencing, we consistently detected the anomalous sequence.
Reamplification and resequencing of the original cell lysates in some cells showing anomaly (about 10%) failed to reproduce the anomalous sequence and the newly determined sequence had the consensus sequence expected for most single cells stemming from the sample.
The anomalous sequence at the 5' end of the rhesus RALY transcript is likely due to this incorrect assignment.
The frequency of cell showing anomalous sequence was thus low (0.6%).
In total, we identified 40 cells with anomalous sequence variation from a total of 7094 single cells by direct sequence comparison, although the negative controls included in these experiments consistently yielded no detectable amplification on 1.5% agarose gel.
Similar(50)
Furthermore, these training sequences have to contain a few anomalous sequences.
No numt or other anomalous sequences were observed.
Sequencing errors should be considered a possibility and the anomalous sequences verified where appropriate.
Database entries for these anomalous sequences are currently under review by NCBI (personal communication).
The disadvantage is that anomaly detection algorithms need a vast quantity of data to detect anomalous sequences based on training sequences.
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