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A kinetics-based model is proposed to explain the anomalous migration behavior and morphology changes of the Ge QDs based on the Si flux generated during the oxidation of Si-containing layers.
When purified HP0593 protein was checked on SDS-PAGE an anomalous migration was observed.
The resultant enzyme showed an anomalous migration in SDS-PAGE, possibly due to its special amino acid composition.
Since T. cruzi Pho1 has 12 transmembrane domains, a size discrepancy between the expected (107 kDa) and the observed molecular mass could be attributed to the usual anomalous migration of hydrophobic protein on SDS gels [51] or to partial degradation.
However, the migration of the less acidic proteins such as GST-only (pI 5.9) and GST-NterTRP47 (pI 5.5) were unchanged after EDC treatment, suggesting numerous acidic residues present on TRP47 and C-terminal TRP47 (GST-CterTRP47) are responsible to a large extent for the anomalous migration of these proteins on SDS-PAGE (Figure 4C and Table 4).
Importantly, some bands may exhibit anomalous migration due to gas vesicle protein resistance to the standard protein dissociation protocols [ 10, 11].
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The driving force for the observed anomalous F migration remains unknown [4].
However, this study has defined the molecular basis for the anomalous electrophoretic migration of immunoreactive, acidic E. chaffeensis TRPs, and determined these proteins are not glycosylated.
MCM2 appears to have an anomalous SDS-PAGE migration profile, as a Mass spectrometric measurement indicates a molecular weight 10,774 Da (data not shown).
This discrepancy may have been due to anomalous gel migration.
Similar reports for anomalous E1 migration in SDS-PAGE have been previously reported [ 16, 23].
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