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Apart from the anomalous difference in this year's matches, there are some trends that have held steady over the last five years.
To examine the presence of Ca2+, we collected a diffraction data set at a wavelength of 1.7000 Å and calculated an anomalous difference Fourier map.
This interpretation is further supported by the anomalous difference Fourier map calculated from dataset K027PKmAChE (Table 1) collected at a wavelength of 0.91944 Å, close to the bromide K-edge.
A clear electron density peak was found at the corresponding site in the LNS5 domain of NX1α III) although no signal was observed in the anomalous difference Fourier map.
The molecular replacement solution was verified and 60 selenium sites were identified via an anomalous difference map using data collected at the peak wavelength for selenium from the SeMet #1 crystals.
Confirmation of the model building for the bridge element was obtained via inspection of an anomalous difference map using data collected at the peak wavelength for selenium from the SeMet #2 crystals.
In an anomalous difference Fourier a slightly larger signal should therefore occur at the Zn2+.
Strong Se peaks in the anomalous difference map contoured at 5 σ (magenta) indicate SeMet positions.
Pink mesh, Br anomalous difference density for Sal Br (contoured at 7.0σ).
(C ) Electron density, Br anomalous difference density, and atomic model for Sal Br.
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Since the AS peptide contains three Met residues, the building of the AS structure was guided by the Se-anomalous difference map as the reference.
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