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To prevent bias from different annotations methods the L. longipalpis and the P. papatasi data sets were re-annotated using the Annocript pipeline with default parameters, obtaining results in general agreement with previous annotations of the two sand fly transcriptomes [ 25, 26].
Among the functional annotations methods applied to the protein coding genes, the Pfam had the highest coverage for all the genomes.
We fitted all the GRM simultaneously using GCTA [ 35], and recorded the heritability (the proportion of genetic variance over the phenotypic variance) and the S.E for each trait across all of our annotations (Methods).
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The common annotations method describes each cluster by listing the top-3 most frequent annotations assigned to at least two experiments in the cluster.
Moreover, Sma3s outperformed other widely used annotation methods when annotating DNA array datasets (Fig. 5).
The identified protein-coding regions are annotated using the high-throughput protein function annotation methods implemented in PIPA [3].
Figure 3 shows the number of transcripts annotated by one or more of the annotation methods.
Using several different annotation methods, approximately 54% of the transcripts were annotated.
We compared the number of reactions and annotated genes (catalysing at least one reaction) identified by the different annotation methods.
Automatic image annotation methods based on searching for correlations require a quality training image dataset.
Therefore, complementary and/or alternative annotation methods are needed to overcome the aforementioned shortcomings of explicit tagging.
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