These resources will allow scientists to query gene expression and functional annotation in response to drought.
Based on GO term assignment and KEGG annotation, 187 assembled unigenes were annotated in "response to stress" under the level 3 classification for "biological process", while 96 assembled unigenes were associated with the pathways of "xenobiotics biodegradation and metabolism" under the KEGG metabolism pathway.
Submissions sent without a reminder were more likely to be missing some annotations than ones submitted in response to a curator email request (at the 99% significance level).
GO annotation identified 1,934 unigenes involved in response to stimulus (GO: 0050896) (Table 2; see Table S1 in Supplementary Material available online at http://dx.doi.org/10.1155/2014/493825).org/10.1155/2014/493825
Automatic functional annotation of PARP-1-dependent genes in response to either anti-CD3 or anti-CD3 + anti-CD28 stimulation was performed based on Ingenuity software.
The next incremental phase of FlyBase annotation involved updating specific gene models in response to new data including cDNAs released by the BDGP (Stapleton et al. 2002), community cDNAs, and gene model updates from individual publications.
Coincidently, suppressed transcripts of both wounds enriched into stress-response pathways, including EIF2 signaling; however, this was more extensive for P.a.-infected wounds, including many-fold more transcripts enriching in the 'cell death' annotation, suggesting resident cutaneous cell toxicity in response to a more damaging P.a.
The DEGs were classified into 33 categories after Gene ontology (GO) annotation, in which 68 genes are in response to stimulus and involved in immune system process, 12 genes are related to cell wall, and 13 genes belong to transcription factors.
Many genes including 4 microsporogenesis, 11 flower development, 13 pollen development, 3 pollen tube growth, 12 pollen germination, 12 pollen exine formation, 9 pollen wall assembly, and 14 sporopollenin biosynthetic process from GO annotation were identified as spikelet genes in response to high temperature interacting with high nitrogen level in ZHN-vs-ZHH (Additional file 15: Table S5).
