The last ten annotations comprised seven sequences with a complete ORF, and three with a prematurely interrupted ORF.
The Gene Ontology (version February 29th , 2006 Cellular Component annotations of each signalling module protein have been slimmed to a list of annotations comprising the 4 following terms: Membrane or Extracellular, Cytoplasm, Nucleus and Other.
Both O. sanctum and O. basilicum annotations comprised of all most all the genes involved in the biosynthesis of essential oil specific phenylproanoids and terpenoids indicating the completeness of transcriptome data (Tables 3, 4 and 5).
Rhythm annotations comprised five types (see [ 21] for further details): VF and fast ventricular tachycardia (VT) in the shockable category and ASY, pulseless electrical activity (PEA), and pulse-generating rhythm (PR) in the nonshockable category.
Annotations, comprising protein coding genes, large and small ribosomal subunit genes, and tRNAs, were completed for each genome utilizing DOGMA (dogma.ccbb.utexas.edu/) [ 20], RNAWeasel (http://megasun.bch.umontreal.ca/cgi-bin/RNAweasel/RNAweaselInterface.pl) [ 21], tRNAscan-SE 1.21 [ 22], BLAST (http://blast.ncbi.nlm.nih.gov/) [ 23], Geneious, and previously annotated genomes.
Gene ontology annotation comprising biological process, molecular function, and cellular component was performed using Blast2go [ 52].
Nevertheless, this gene annotation comprises many incomplete gene models (14%) without start and/or stop codons.
Functional annotation comprised of homology based analysis of all sequences in the Phytozome soybean transcriptome.
The pipeline for functional annotation comprised four main steps: 1) data acquisition 2) sequence/data cleansing, 3) contig assembly and 4) BLAST plus putative functional annotation.
