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To avoid redundant gene modeling, a filtering step was included with the E-value, confidence of assembled contigs and length of the contigs as the thresholds to select the top hit contig for the unique annotation name.
For reaction elements, attribute information about annotation, name, reactants and products for each reaction of the first SBML model are compared and matched to those of the reactions in the second SBML model, in order to identify whether the two SBML models share common reactions.
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In the first type of random annotation, named random1, the original sets of protein annotations were randomly redistributed among the proteins.
For the second type of random annotation, named random2, a new PPI-network was constructed by placing an edge between two proteins with probability equal to the fraction of edges in the original graph.
bAdditional early annotation names of the same protein.
Annotation names from the GO analysis represent the top BLAST hit (Additional file 2).
So these three genes were only listed gene code numbers without annotation names.
Because gene annotation is currently labour intensive, we used a locally developed platform dedicated to phylogenetic annotation (named FIGENIX) [ 6].
They range from simple data (de)serialization to natural language processing (NLP) to semantic annotation (named entity and relationship recognition).
In sum, we were able to assign "custom annotation" names for 19,098 sequences, or 60% of the Build 3 assembly.
Additionally, this analysis identified 403 putative protein-coding genes not covered by the current annotation (see " Geneset annotation: naming of genes and geneset comparisons below).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com