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The filtered genes were annotated using a chicken annotation file (Chicken. na32.annot, Affymetrix, USA).
Genes were annotated using the lncRNAs annotation file coming from Gencode (release 19).
The rHH regions were annotated using the Illumina 1M annotation file (Human Genome build 36.1 RefSeq).
To annotate the probes in the Affymetrix array we used the latest annotation file available [ 17].
-j, -junction-file Annotation file for known exon junctions.
For the gene information, we first downloaded the gene annotation file from Solgenomics (ftp://ftp.solgenomics.net/genomes/Solanum_lycopersicum/annotation/ITAG2.4_release/).net/genomes/Solanum_lycopersicum/annotation/ITAG2.4_release/
We used the processed annotation file and sequence features for mouse transcripts (mm10 GRCm38.p5, ANNO_FILE = SE.gold, FACTOR_FILE = mouse_factors.SE.gold).SE.gold
The output file can be used as junction annotation file for OLego (option -j).
This file can be directly used as junction annotation file for olego.
Note that if junction annotation file is provided by -j, these "known" junctions will still be searched.
The number of reads that aligned to each gene was counted using HTSeq-count27 and an annotation file that contained only poly-adenylated transcripts.
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