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The relatively low number of reads mapping to gene-annotated regions (20%) accounts for the low annotation coverage available for the sheep genome.
As modules M2 and M3 only annotate query sequences that were not annotated by the previous module(s), the ∼26% of sequences annotated by M3 suggests that it significantly increases Sma3s annotation coverage.
The updated annotations have a considerable impact on the annotation coverage for these sets of genes, with the un-annotated fraction dropping from ∼35%to∼16%6% for all the tissue comparisons.
We show that the results of MITOS match RefSeq and MitoZoa in terms of annotation coverage and quality.
Functional annotation charts were used to retrieve an extended annotation coverage that included more than 40 annotation categories [15].
Currently, sequences deposited in public databases are automatically reviewed with increasing accuracy and annotation coverage is nearly 60% of the total proteins (as of November 2008).
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A key improvement in this revised annotation is coverage of untranslated region (UTR) sequences, with 79%and59%9% of transcripts containing 5' and 3' UTRs, respectively.
Most of the differences in gene content correspond to differences in copy number within gene families and others that can be attributable to differences in annotation or coverage between the two assemblies (supplementary table S1, Supplementary Material online).
Comparisons against reference data sets (CEGMA core genes, BUSCOs, and ubiquitously expressed genes) showed that the P a. guttatus and C. ocellatus transcriptomes are the most complete, whereas the ones of S. punctatus and P. molurus present the lowest annotation and coverage.
For gene-variant relations, they found that their SNPshot method recovered 96.5% of the PharmGKB gene-variant annotations, after processing nearly 180 000 PubMed abstracts, though for gene-RefSNP annotations the coverage dropped to 65.4%.
Putative transposable elements were identified by BLASTX searches against the Arabidopsis thaliana proteome (TAIR10) with an E-value cut-off of 1E-4, ProDom annotations and coverage analysis with the Silene repeated sequences database [ 37].
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