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The gene ontology annotation completed in this study is an excellent source of information for future investigations of this parasite.
Once the contigs were built and their annotation completed, all data were loaded in a locally-adapted Ensembl database.
They can then claim 40-kb projects posted on the GEP website and submit the results of the finishing and/or annotation completed by their students.
The genomes of both of these organisms have been sequenced, with the M. ulcerans Agy99 genome annotation completed and the M. marinum M genome sequence in the process of being annotated (Table 1).
Currently, the interface provides all of the annotation details for the studies with annotation completed by all biocurators, as the number of biocurators is limited to our lab at the time of writing this paper.
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Annotation of coding sequences for isolate 08-5578 was completed using GenDB v2.2 [ 63], BASys [ 64], Glimmer3 [ 65] and by comparison to RefSeq annotations completed for L. monocytogenes strain EGDe [ 22].
Three annotators completed the annotation of the figure-caption pairs in 180 hours.
Annotation was completed using the genome annotation system GenDB [ 17], and manually optimized.
Once the entity annotation is completed, we have conducted relation annotation with the help of WebAnno copy annotator.
For each unigene cluster, the section 'Sequences' was screened for mRNA sequence information and mRNA sequence information (e.g gene identification (id) number, gene annotation, complete cDNA sequence (cds) or not) was retrieved and analyzed.
Using BLAST for functional annotation, complete lists with putative gene identifications for these EST clusters are provided in Additional file 1.
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