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Likely coding regions (Open Reading Frame) in the transcripts were identified using Transdecoder, which is an application within the Trinity software version trinityrnaseq_r2013_08_14. High quality transcripts were annotated with the BLAST2GO [ 38], a comprehensive suit designed for the functional annotation and analysis of gene and protein sequences.
The predicted proteins were further annotated with the Blast2GO pipeline12 to assign GO and GOslim-plant terms based on NCBI plant databases and InterProScan34.
The topics identified through TM were annotated with the 12 themes by a small group of observers.
Contigs and isotigs were annotated with the Blast2GO software.
This resulted in only 10 sequences being annotated with the IPR001092 signature.
On the other hand, two thirds of the homologous transcripts annotated with the Uniref100 data base had an unknown function.
Before assembly, the reference genome was annotated with the 384 amplicon targets, consisting of 382 discontiguous regions, using a gff file (Additional file 1: Table S2).
Conserved and Brassica-specific miRNA precursors were locally blasted with Brasscia genome and the best hit positions were annotated with the gff3 format.
The translated protein sequences of both the corrected B. floridae CDSs and the assembled A. lucayanum CDSs were annotated with the Blast2GO pipeline (Conesa et al. 2005).
We found eight genes that harbor the replicating SNP-SNP models that were annotated with the PI3K/Akt pathway group (Fig. 5B).
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