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Each EST was annotated with the top human RefSeq hit.
Complete contigs containing SNPs compared (BLASTX) to CDD and Swissprot databases and annotated with the top BLASTX hit if the database match had an e-value of < 10-10.
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Each gene that has 7302R-specific SNP was functionally annotated with the GO annotation data, and the top 10 GOs were listed.
The resulting top-20 words per topic were annotated with the twelve big data themes by seven observers.
They are all heavily annotated, with the most gruesome passages all dutifully underlined.
Finally, we mapped each SNP to the appropriate Broad bin and annotated each with the top 18 PCs for purposes of the analysis.
Contigs were annotated with their top human RefSeq (3/2006) hit using discontiguous Mega BLAST and the following options -m 8 -D 2 -t 21 -W 11 -q -3 -r 2 -G 5 -E 2 -e 1e-5 -N 2 -F "m D" -U T -v 5 -b 5. Discontiguous Mega BLAST, which is based on the use of discontiguous templates [ 24], is designed for alignments of diverged sequences, most notably sequences from different organisms.
Figure 2 presents, as an example, a diagram of network 1 that shows specific neurodevelopmental functions of the NPE, annotated with top functions "Embryonic, organ and organismal development".
Mean and median values of these metrics are also annotated on the top of the plots.
In addition, the color schema for the values is annotated on the top of the table.
More than 80% of unigenes could be annotated with sequences from the nine top-hit species.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com