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The resulting fragments were annotated on the basis of the current genome annotation and fragments containing sequences of adjacent annotated genes were removed.
(2) In the framework of the human and vertebrate analysis and annotation initiative (HAVANA) [ 21], where gene structures are annotated on the basis of human interpretation of combined supportive evidence generated during sequence analysis, we manually annotated seven DEF gene containing clones, five of them located in DEF cluster a.
Of the 895 contigs from the A. americanum immuno-transcriptome, 431 (listed in an Additional file 1) did not show amino acid identities to previously annotated proteins in GenBank, while the remaining 464 sequences were provisionally annotated on the basis of their identity to protein sequences in GenBank.
For genes encoding mitochondrial ribosomal proteins, 6 could be annotated on the basis of the co-coexpression analysis (4 confirmed PlasmoDB v5.4 annotation, 1 refinement of an incomplete annotation and 1 functional inference of hypothetical genes).
Filtering for genes annotated on the basis of experimental evidence only, as described above, reduced the list to 77 genes.
Gene products were annotated on the basis of similar proteins of Am. methanolica and Ko.
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This article is also annotated on the site.
Approximately 50% of these have been annotated, largely on the basis of known functions in Drosophila melanogaster [40].
The potential for predicting protein function similarly rests on its inference over incompletely annotated sequences on the basis of homologues in other species (Carroll and Pavlovic, 2006).
The CC2D1A protein contains four DM14 domains that in turn are annotated only on the basis of amino acid sequence comparisons, but their biochemical and cellular functions have remained elusive [ 20, 27].
Although its genome sequence has been resolved more than a decade ago [ 28], approximately half of its ca. 30.000 genes remain functionally unknown or are annotated only on the basis of static analyses such as protein motifs or similarities [ 29].
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