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Many of the conserved ORFs newly identified in one strain were homologs of genes annotated in the second strain (48 ORFs newly identified in 2CP-C and 25 in 2CP-1) and most were functionally characterized (Supplementary Tables S16 and S17).
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Then the three annotators started two rounds of annotation, where 100 documents were annotated in the first round and 150 in the second.
Those not annotated in the first step were then submitted for similar annotation process with UniprotKB human proteome and NCBI nonredundant protein sequence database.
In a preliminary analysis, 12 genes were annotated in the first category photosynthesis in both MchloDSM and MchuDSM whereas none was found either in MycNBB4 or in MobuDSM.
The second variant annotated in the human PGF assembly (OTTHUMT00000076177) contains three additional exons, one of which cannot be resolved in gorilla (between gorilla exons 19 and 20).
This alternative 3'SS (not annotated in the TAIR10 Arabidopsis genome) extends the third exon into the next intron and thus generates a larger exon.
With only the IFITM3-like, IFITM5 and part of the IFITM10 genes annotated in the chicken genome we had to first identify the genomic locations of other chicken IFITM genes (if any) and identify the orthologous genes on the relevant scaffolds in the sequenced duck genome.
First, DHH is not annotated in the canine genome so primers were designed based on a region of the canine genome homologous to the gene in other species.
First, we used SNPduo++ to find identical samples from those that were annotated in the pedigree file as non-identical.
Relevant information was annotated in the MINT database.
There are six OsRUS genes annotated in the rice genome.
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