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Based on GO term assignment and KEGG annotation, 187 assembled unigenes were annotated in "response to stress" under the level 3 classification for "biological process", while 96 assembled unigenes were associated with the pathways of "xenobiotics biodegradation and metabolism" under the KEGG metabolism pathway.
Approximately half of the genes annotated in " Response to organic substance" were also genes annotated as apoptotic genes.
Therefore, two predicted members of this family, PUB23 and CMPG1, which are among the DEGs in our experiment and were annotated in response to chitin and/or death, were also analysed by in situ qPCR.
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Only DEGs annotated in the Defense response (GO 0006952) biological process and/or child ontologies related to defense are shown.
For the known genes in a GRM that have not been annotated in heat shock response, most of them are related to one of the cellular processes mentioned above.
However, at least for identifying strongly induced genes, short read sequencing approaches appear to be robust and accurate, suggesting that this approach may prove to be a simple and cost-effective way to identify differentially regulated genes in poorly annotated genomes in response to any number of treatments of biological interest.
To identify putative Glycine max ARF target genes for the most abundant TAS3 tasiRNAs, alignments to 12 Glyma model sequences annotated in Phytozome as auxin response factors (ARF) were examined.
These genes function in different levels of the biotic stress defence response signalling pathway and were annotated in biological processes such as the response to chitin and/or cell death (Table 3).
We selected genes annotated in different biological processes (biotic defense response, signaling transduction, phytohormone signaling and transcriptional regulation).
Genes identified and annotated in this study include candidates for responses of the blue crab to xenobiotic compounds, which could serve as biomarkers for oil exposure.
Authors' response: We acknowledge that these examples are already annotated in many genomes with the associated function.
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