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The Ensembl database, however, does not have this antisense transcript annotated but it does have two genes annotated further downstream as shown in the Ensembl annotation track in Figure 4.
Color-specific and proximal-distal gene lists were annotated further.
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However, we could annotate further 15 UniTags to at least six other Fb classes.
Transcription start sites were annotated and further analysed according to their position relative to annotated genes.
None of the dozen other genes in the vicinity are annotated, so further fine structure mapping and molecular genetic analyses will be required to establish the identity of the gene that causes the cardiac failure.
Moreover, the KEGG pathways of upregulated DEGs were annotated for further understanding participant metabolic and cellular processes.
Matches to InterPro signatures of protein domains, the majority of which are well annotated, provide further hints describing the likely biological roles of genes with recognizable sequence signatures.
When compared against the InterPro domain database, 497 of these CDS were successfully annotated, and further assigned to Gene Ontology terms.
BLASTX searches produced 3,243 significant (E-value < e-10) hits and Gene Ontology (GO) analysis annotated a further 1,223 of these genes (37.7%).
We manually annotated a further 4727 proteins into non-chromatin and chromatin classes, the latter including functional subcategories ("Lab internal category" in Supplementary Table 1).
Among the 37 DEGs, 19 DEGs have a high degree of sequence similarity to known Arabidopsis orthologs; these were annotated and further analyzed (Table 2).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com