However, they still remain strongly affected by sequencing and annotating errors [ 10].
We correct previous annotation errors and annotate new genes, resulting in the identification of 51 genes in Arabidopsis that encode LEA proteins.
One annotation error concerns two chemicals after each other that were annotated as one in the sentence "On interaction with anhydrous potassium acetate 14-bromcarminomycinone (III) yield 14-acetoxycarminomycinone (IV)".
Moreover, similar to Le et al. (2011) and Liu and Golparvar-Fard (2015), each contributor starting with a project that is already annotated without an error and educate them by presenting falsely labeled patches with the ground truth can be used to present consistent mistakes observed in this study.
Any discrepancies between the data in the original JCAMP file and the process data are annotated in the errors element, with details of the issues.
We also report genome annotation errors for two proteins, expression of ORFs annotated pseudogenes for four proteins and one correction to the sequence of another previously annotated pseudogene which allows for its full length expression.
Others are due to: Prodigal annotation errors, e.g. split gene models; genes annotated as pseudogenes in MG1655; genes missed by the syntenic gene pairs assignment algorithm.
We provide experimental evidence of expression of 5 genes that were originally annotated as non-expressed pseudogenes, as well as start site annotation errors for 2 other genes.
In our study, the detection of annotation errors, such as incorrect start sites assignment, sequencing errors, and wrongly annotated pseudogenes, would prevent misannotation from being multiplied in future versions of the S. flexneri 2a str.301 genome.
To control for potential annotation errors, we used a subset of overlapping genes that were not annotated as "hypothetical," "putative" or "pseudogene" in the NCBI genome data.
