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Following collection animals were terminated by cervical dislocation.
Following 10 mins of insulin stimulation, animals were terminated by cervical dislocation and EDL and SOL muscles were rapidly removed, rinsed in PBS and snap frozen in liquid nitrogen before being stored at −80°C.
Following muscle collection, animals were terminated by cervical dislocation.
Seven days after surgery, animals were terminated by anesthetic overdose and the nerves collected at −80°C.
The animals were terminated by an overdose of sodium pentobarbitone (100 200 mg/kg) IV after ketamine sedation.
Both tumour volume and body weight were monitored daily and animals were terminated by cervical dislocation when the body weight loss reached 25%.
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Animals were terminated at 5 (n = 5) and 6 (n = 3) weeks postoperatively.
The animals were terminated at 78 hours.
After the scanning period, the anaesthetized animals were terminated.
All animals were terminated at the appropriate time using an overdose of halothane (Concord Pharmaceuticals Ltd., Essex, UK) followed by cervical dislocation.
Animals were terminated 60 h after cell injection and samples collected for subsequent analysis.
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