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In the chronic stimulation experiments, all animals were stimulated daily for 1 hr during 14 00 19 00 hr.
Starting 2 weeks after the HI event, animals were stimulated by the enriched environment (1 h/day for 9 weeks).
Animals were stimulated (16 Hz sinusoidal light protocol, ~4 mW laser power) when the body center point passed from the first zone (Wall) into the Intermediate zone (surrounding the petri dish).
In the second experiment, treated animals were stimulated (amplitude: 1.5 mA; frequency: 30 Hz; pulse duration: 500 μs; on/off time cycle: 30 s/5 min) for 3 h per day, during five consecutive days.
Animals were stimulated for 3 seconds with various odors (benzaldehyde, iso-amyl acetate, ethylpropionate) at saturated vapor pressure.
For the LTP study, animals were stimulated at Schaffer collaterals every 20 s for ≥15 min, until a stable fEPSP was recorded (baseline, Fig. 4A).
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CCM formation in resistant Ccm2ECKO animals is stimulated by abscess formation and LPS.
"Mankind is everywhere the same," she writes to her daughter, then adds: "This observation might be carried yet further: all animals are stimulated by the same passions, and act very near alike, as far as we are capable of observing them".
However, in the present study cows with mastitis were in different stages of lactation and had significantly higher levels of insulin than the rest of the cows indicating that the pancreas in these animals was stimulated by more glucose eventually due to decreased glucose drainage to the udder.
For baseline and post-injury measures, each animal was stimulated five times bilaterally with each filament.
One week after the last infusion, electrodes were positioned on the neck over the vagus nerve and the animal was stimulated for 1min.
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