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Animals were provided with tap water ad libitum and standard pellet diet.
All animals were provided with standard food and water ad libitum and were exposed to alternating 12-h periods of light and darkness.
Animals were provided with food and tap water ad libitum and allowed a 1-week acclimation period after arrival to the animal facilities of the Faculty of Pharmacy.
Animals were provided with continuous maintenance intravenous fluid resuscitation and boluses as needed with Lactated Ringers to maintain a mean arterial pressure (MAP) >65 mmHg.
All animals were provided with unchopped teff (Eragrostis tef) straw ad libitum and were supplemented with either 150 g of wheat bran (control), leucaena (200 or 400 g) or sesbania (200 or 400 g) leaves for a period of 6 months.
All animals were provided with chow and water ad libitum.
Injected animals were provided with 0.16% Neomycin (Sigma-Aldrich -supplemented water.
Animals were provided with environmental enrichment designed by the clinical veterinarian.
During the night the animals were provided with water ad libitum, banana, gum of Acacia trees (if available) and mealworms.
All animals were provided with a highly detailed certificate and were routinely checked for infection with more than 40 infectious agents, including 24 bacteria, 12 viruses, 4 parasites and 3 fungi.
They were housed in 50×30×70 cm individual glass vivaria where the floor was covered with soil; in the vivaria the animals were provided with an intricate light-proof plaster structure for shelter.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com