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Animals were maintained seven weeks before sampling pituitary glands.
Animals were maintained in accordance with the guidelines of the UCLA Animal Research Committee.
All the animals were maintained under conventional conditions with food and water ad libitum.
Animals were maintained in a pathogen-free barrier facility at the University of Barcelona animal facility.
All animals were maintained in specific pathogen-free facility at Yale Animal Resource center (YARC).
All animals were maintained for at least 20 generations in the C57BL/6 background.
Throughout the imaging, animals were maintained under anesthesia with 1.5% vaporized isofluorane.
Animals were maintained with free access to food and water ad libitum.
During hg, the animals were maintained in aquarium water containing alizarin-complexone (AC), a fluorescent calcium tracer.
Restrained animals were maintained in plastic cylinders for 22 h/day.
Control animals were maintained on a normal diet and water for the same period.
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