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The animals were dissected to examine the contents of their stomachs, although the results of the autopsy procedures were not released.
Briefly, brains from control and treated 2 wpl animals were dissected out and immediately incubated in 150 µL of phosphate buffered saline (PBS) containing 1 μL of the 150× FLICA labelling solution at 37 °C for 1 h.
Animals were dissected after imaging.
At the time of sacrifice, all animals were dissected systematically by the same person, and the number and location of metastatic sites were noted.
Brains of sacrificed animals were dissected, snap-frozen in liquid nitrogen, and stored at −80°C.
Animals were dissected at L4+24 hours, fixed in 3% paraformaldehyde and 0.1 M potassium phosphate for one hour, and stained with DAPI.
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The hippocampus, excluding most of the subiculum, from two to three animals was dissected and pooled for hybridization to a single Affymetrix M430 2.0 array.
For measurement of muscle weights, individual muscles from both sides of the animal were dissected, and the average weight was used for each muscle.
Both knee joints of each animal were dissected, and the femoral condyles were fixed in PBS (pH 7.4) containing 4% paraformaldehyde, decalcified in 12.5% EDTA, dehydrated and embedded in paraffin blocks.
Single coronary arterioles (1 vessel from each animal) were dissected from the heart of Wistar rats.
Hind limbs from each animal were dissected and fixed in neutral buffered formalin overnight at +4°C.
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