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The F1 animals were checked.
The F1 animals were checked with Zeiss LSM710 META confocal microscope.
Animals were checked for the body weight, food and water intake on a daily basis.
Further, the operated areas and general conditions of the animals were checked daily according to standard veterinary postoperative care.
The animals were checked in the MRI system every 3 to 5 days after intracranial surgery to monitor the tumour development.
Although, all rabbits responded well to the repetitive immunizations, however in order to select the most appropriate animal for scarifying and collecting the total sera, the affinity of these antisera from all animals were checked by competitive ELISA as described above.
Animals were checked daily for neurological signs.
Animals were checked daily by qualified personnel in the lab.
Animals were checked twice weekly for the development of palpable tumors at the site of injection.
Additionally, the body weights of all animals were checked weekly for 8 weeks.
In addition, the glomerular basement membranes (GBMs) of the immunized animals and control animals were checked by electron microscopy.
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