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Multiple sequence traces from control and case animals were aligned and compared using the phredPhrap program from the Consed package (www.genome.washington.edu).edu
Next, a total of 5,773 sequence sets corresponding to α1 domains from Ascomycota and HMG domains from fungi, plants and animals were aligned with the core region using Muscle [18] and conserved sequences identified.
The read sets obtained by sequencing 20 animals were aligned against the Gallus gallus WASHUC2.1 genome reference obtained from Ensembl 58 using BWA v0.7.0 (Li and Durbin 2009).
Full length HMG domain sequences (79 amino acids) encoded by the Sox genes of Acropora and other animals were aligned by ClustalW prior to Maximum Likelihood phylogenetic analyses in MOLPHY version 2.3 [ 49] using the Dayhoff model of protein evolution and local rearrangement of the Neighbor-joining tree.
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DNA sequences from all the positive tissues belonging to an individual animal were aligned, and consensus sequences representing each animal were created.
Mature and precursor sequences of miRNAs across animal species were aligned with Clustal X 2.0 [35] respectively by using the multiple alignment.
The PBV nucleotide sequences of different animal species were aligned using ClustalW with human PBVs along with GG-I and GG-II reference strains.
For each animal, reads were aligned against the 26 gene sequences encoding paralogs of FFAR2 identified in 2011 by Ensembl, using BWA software (Li and Durbin 2009).
For each animal, the scan series were aligned to the postoperative scan using rigid registration, with a minimum correlation coefficient of 0.8, convergence criterion of 1 × 10−5, and maximum 2000 iterations [ 17, 18].
When NAC was conjugated with animal cap, the polarized cells were aligned perpendicular to the boundary, as described above (Fig. 4g).
The squares were used to indicate the location of animals during the test: eight squares were aligned with the edge of the walls (the peripheral area) and one square was inside the outer squares (the center area).
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