Animals were administered by means of intraperitoneal (i.p).
Animals were administered analgesics postoperatively, and monitored during the recovery period until their mobility returned.
Postoperatively, the animals were administered 1 g of amoxicillin once daily for 5 days.
All animals were administered 5 μg/kg/min Escherichia coli lipopolysaccharide (LPS).
All animals were administered 5 μg/kg/min Escherichia coli lipopolysaccharide (LPS) (serotype 055 B5; Sigma Chemical Co., St . Louis MO, USA).
In experiment two, animals were administered either the estrogen synthesis inhibitor, fadrozole, or vehicle, and the syrinx measurements were taken at day 60.
In sub-acute toxicity studies, both male and female animals were administered with extract daily for 14 days and were sacrificed for hematological, biochemical and histological examination.
Group I: Animals were administered a single dose of distil water (1 ml/kg, p.o). daily for 5 days and received olive oil (1 ml/kg, p. o). for another five days.
Once at steady state, animals were administered a second dose of 18F-NaF (15.5 ± 0.1.7 MBq/kg, n = 4) IV, followed by repeat collection of cardiac output and heart rate in triplicate.
Immediately after the administration of each anticoagulant, the animals were administered 50.0 mg/kg of histone H3 (Calf thymus histone H3, Sigma-Aldrich, Co. (St . Louis MO, USA)) by intravenous injection.
To sustain the growth of the ER-positive ZR-75-1 cell model, animals were administered a 1.7 mg, 60-day slow-release estrogen pellet from Innovative Research of America (Sarasota, FL) subcutaneously in the dorsal space of the neck.
