Structure and evolution of LEC (purple, n = 3 animals), CA3 (blue, n = 3 animals) or MEC (gold, n = 2 animals) activity, quantified by applying several distance measurements to population activity states (see Supplementary Information).
Figure 4 shows microscopic preparations of representative vaginae from one animal per treatment group; the morphologic findings in vaginae of all animals were quantified and are presented in Table 3. Atrophic vaginal epithelium was observed in control animals; in 12 of 12 rats, only two to three cell layers were present, and these were composed of flattened cells with no cornification.
To determine if UCP1 expression is altered in PRLR KO animals, we quantified UCP1 expression in interscapular BAT.
The D-type neuron phenotype of L1 stage animals were quantified 10 hours later after lysing the adult heterozygous mutants, using alkaline hypochlorite.
To determine whether adiponectin deficiency had any effect on the metastatic ability of B16F10 cells, we injected the B16F10 cells into mice through the tail vein, and did not observe a significant difference in lung colonization between the adiponectin deficient mice and the wild-type control animals, as quantified by counting lung nodules in individual mice (Figure 1D, p>0.05).
In an attempt to separate these effects, we infected mice with H. pylori cultured for 12 h with or without 1% DFMO prior to inoculation, in the absence of in vivo treatment of the animals, and quantified bacterial colonization after 1 month by homogenizing a portion of the stomach and plating dilutions on solid medium.
20 30 animals were quantified per group, per experiment.
The velocity of animals was quantified using worm tracker software (Ramot et al., 2008).
The morphologic findings in uteri of all animals were quantified and are presented in Table 2.
Selected cDNA samples from seven of the eight animals were quantified by real time qRT-PCR.
