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All animal procedures were approved by the Animal Ethical Committee in accordance with the Guidelines for the Care and Use of Laboratory Animals prepared by this university.
We considered the full cohort of animals prepared for a given study to be the 'universe' of animals from which the smaller groups were drawn randomly using sampling without replacement.
RNA was initially qualified using a standard spectrophotometer and a pool of 6 animals prepared for library production.
Investigators adhered to the Guide for the Care and Use of Laboratory Animals prepared by the National Research Council.
The samples were obtained from animals prepared in the same manner as described above for GnRH expression and sacrificed at 60 min following E2B treatment.
Research with animals was conducted according to the principles enunciated in the Guide for the Care and Use of Laboratory Animals prepared by the Institute of Laboratory Animal Resources, National Research Council.
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