For animals used for collection of cauda epididymal sperm for motility analysis, two animals were used at each time point.
In addition, capture or killing of animals for specimen collection may result in the rapid dispersal of contagious animals [ 86].
Serologic analysis was not feasible at the time of the outbreak due to the need to use anesthesia to immobilize the animals for blood collection.
Two days after arrival, animals were euthanized for collection of bone marrow.
Although this suggests that only a very small injury to the body (i.e., blood withdrawal) is needed to collect donor cells, there remains the risk of accidental death by injury caused by the need to restrain the animal for blood collection.
Animals were serially killed for collection of plasma and lung tissue at 11 time points over 96 h after exposure.
Growth of tumours to volumes of 1 - 1.5 cm3 was taken as evidence of successful xenografting, and the animals were then sacrificed for collection of tumour tissues for further studies.
Led by the criminal zoologist Lutz Heck, they carted off the best animals for their own collections.
The protocol for animals and collection of blood samples was approved by the Virginia Tech Animal Care and Use Committee (IACUC).
Table 1 Location, number of individual urodeles, number of species sampled and the number and proportion of Bsal-positive animals for each private amphibian collection at the time of testing.
