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Fluid specimens are typically whole animals fixed in Formalin and stored in 70% ethanol.
The brains were excised from the animals, fixed in 10% buffered formaldehyde and embedded in paraffin.
Lungs were removed from euthanized animals, fixed in 4% buffered formaline for at least 24 h at 4°C, and then embedded in paraffin.
Biopsies of the kidney were taken directly after euthanasia of the animals, fixed in 4% formaldehyde and embedded in paraffin.
Hearts were removed from freshly killed animals, fixed in 4% paraformaldehyde in PBS at 4°C overnight, and cyroprotected with 30% sucrose in PBS.
The various tissues obtained from experimental animals fixed in 10% formol-saline were dehydrated in graded alcohol, embedded in paraffin, and cut into 4 5 μm thick sections.
Similar(52)
A piece of the quadriceps femoris muscle was collected from each animal, fixed in 10 v/v% neutral buffered formalin, and embedded in paraffin.
Liver tissue samples were taken from each animal, fixed in 10%% neutral formalin and after histological process of dehydration, tissue embedded in paraffin, section with a microtome set at a thickness of 5 6 μm.
Samples from sciatic nerves of selected animals were fixed in glutaraldehyde, post-fixed with osmium tetroxide and embedded in epoxy resin.
After loss of reflexes, animals were fixed in a stereotaxic frame (David Kopf instruments; Nr: 1900).
Animals were fixed in 60% isopropanol on a rotating rack for 20 min.
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