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These animals correspond to those used as controls (basal transcriptome) in Marques et al. [ 8].
A first requisite for the disentanglement of the role of MMPs in GONs, would be to assess whether MMP expression patterns in animals correspond to what has been observed in humans.
The results presented here for the F0, F1 and F2 animals correspond to a test for resistance to E. tenella at the AFSSA Parasitology Unit of Ploufragan following strictly the same challenge protocol.
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It has been reported that the major Mecp2 isoform expressed in the postnatal brain is Mecp2-E1 [16]; hourver, our analysis showed that the main isoform expressed in colon tissue of adult animals corresponds to Mecp2-E2.
The lifespan of the control group of animals corresponded to the mean for the strain ofrat used (Harlan Sprague-Dawley), but as is frequently the case with most mammals,including humans [55], males on average died before females, except for some female treatmentgroups.
The average degree of sparsity by number of selected animals corresponded to the average number of contributors for a given animal in a given size situation.
The mammals belonged to 11 species, 75.1% of the captured animals corresponded to the Sigmodontinae subfamily, 23.7% to the Murinae subfamily, 0.6% to the Caviidae family, and 0.6% to the Didelphidae family (Table 1).
Our RNA-Seq data reveal that the relative representation of dhs-3 mRNA in GSC animals corresponds to the expectation for a somatic gene that is not regulated by GSCs (see discussion of somatic-specific mRNAs in Figure 3 figure supplement 1).
The 1% CLA diet in suckling animals corresponded to a daily administration of 1.5 mg CLA oil provided/g rat from day 1 to 21. Low-capacity syringes (Hamilton Bonaduz AG) adapted to oral 25- or 23-gauge gavage tubes, 27 mm in length (ASICO) were used for oral administration before and after day 5, respectively.
We observe similar trends in mouse kidney tissue, which was previously found to contain intermediate levels of hmC.[ 5a] Here too, a low hmC level in young animals corresponds to a high fC level, while higher hmC levels during later developmental stages go hand in hand with low fC values.
The applied doses of Neurexan (0.25, 0.50, or 1 tablet per animal) correspond to the 6- to 12-fold human equivalent dosage [ 26].
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