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Ultrathin (≤50 nm) sections were cut from five to eight tissue blocks per animal, mounted onto uncoated 600-mesh copper grids, and stained with uranyl acetate and lead citrate (Ultrostain, Leica, Glattbrugg, Switzerland).
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In plants, although NO research is more recent than in animal, mounting evidences suggest that NO plays important roles in diverse physiological processes such as growth and development (Laxalt et al. 1997), plant disease resistance (Noritake et al. 1996), abiotic stress (Delledonne et al. 1998), and signal transition (Durner et al. 1998).
This configuration provides large needle-accessible areas and enables simultaneous animal mounting for three independent experiments with large populations.
We introduced an estrous female in the home cage of sexually naïve cGαo-/ male residents and found high levels of all measures of mounting, including mounting animals, number of mounts per animal, mounting duration, and latency to first mount, that were not significantly different from those of the B6 control males (t-test: P = 0.49 to 0.96; Figure 5A-D).
LMP7-deficient animals mounted a strong Th2 response during ovalbumin-induced but not house dust mite-induced acute asthma [53].
Serum titers of 102 to 103 were observed in more than 50% of animals in that group, whereas fewer animals mounted an anti-RT response in the RT-ODC gene immunized group.
The Cabela's store in Rogers, Minn., at 185,000 square feet, features a three-story mountain with nearly 400 animals mounted on display, plus aquariums with 55,000 gallons of water, two shooting ranges and an archery range.
The animals mounted a mild hemodynamic response to the double hit.
Although enhanced disease susceptibility proved to be true, mutant animals mounted a surprisingly normal type-I IFN response and fully controlled NSV replication in the CNS.
The GFP LGG-1 reporter is also sensitive to the type of anesthetics used to mount the transGFP LGG-1mals; vereporterGG-1-puncta are observed is alsoalsensitive in 0.1% NaN3, whereas levamisole or tothemisole hydrochloride botypeause noficeanesthetics of puseda witoin 5–10 mountf mountheg transgenics (Fig. 7animals
In this study, all vaccinated animals mounted robust anamnestic antibody responses to epitopes on the challenge viruses, and this may play a critical role in abrogating virus replication and preventing disease.
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