We counted 'freezing' by observing the animal behaviors frame by frame based on the recorded video, the temporal resolution for the video is 30 frames per second and the absolute amount of time that the mice stayed in the continuous frozen state was summed and compared to the total amount of time during which the mice were not in the non-frozen state.
The system was composed of three main parts, including one small animal stereotactic frame, one imaging-fusion software and an automatic robot arm system.
A little bit of everything... for example my current "Masked" series explores the projection of certain character elements by the wearer of the given animal mask framed by a given cultural context.
Meals such as cuy chactado are always served as part of these festivities, and the killing and serving of the animal is framed by some communities as a symbolic satire of local politicians or important figures.
Six to eight week-old GM1 mice were anesthetized by intraperitoneal injection of ketamine (125 mg/kg) and xylazine (12.5 mg/kg) in 0.9% saline, and placed in a small animal stereotaxic frame (Stoelting, Wood Dale, IL).
With the exception of one recording, all running animals display frames with an areal phase (underscored).
Rats were anesthetized with 5 vol% isoflurane and placed in a small animal stereotaxic frame assembly (David Kopf Instruments, Tujunga, CA).
The glioblastoma U87 cells overexpressing PP6c or knocking-down PP6c were intracranially injected into the right caudate nucleus of the athymic nude mice (1 × 10 each), with the aid of a small animal stereotactic frame, and 72 mice were injected.
In the old city zoos — at the height of what might be called zookeeping's Colonial Era — the very harshness of the animals' anthropocentric framing was a direct reflection both of the depth of our awe over their fearsome otherness and of our complete ignorance of their rightful homes.
