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Five brain sections were selected from each animal and processed for counting.
To determine the number of DCX- or BrdU-positive cells in the hippocampus, every 12th section (480-µm intervals) of one cerebral hemisphere was selected from each animal and processed for immunohistochemistry.
The animals were sacrificed after either 1 week, 15 days or 1 month from treatment, the stimulated areas or the MG implants were removed from the animal and processed for electron microscopy or embedded in Polyfreeze tissue freezing medium (Polysciences, Eppelheim, Germany) and immediately frozen in liquid nitrogen.
To determine the number of BrdU-positive or DCX-positive cells in the SGZ of the hippocampus, every sixth section (200- μm interval) of the left hemisphere was selected from each animal and processed for immunohistochemistry.
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These macronutrients in turn can be profitably transformed into animal protein (by feeding them to animals) and processed foods of every description.
LS prepared animals and processed tissues for morphological exams.
Blood, tumour and brain tissue were removed from three untreated control animals and processed in the same way.
Gill tissues from 6 different animals were dissected from -80°C preserved animals and processed for total RNA extraction using the RiboPure tm) kit (Ambion(r), Austin, TX).
Following terminal anaesthesia with CO2 and cervical dislocation, tissues were collected from the animals and processed as required to obtain the different cell cultures.
At days 0 and 5, RNA was extracted from the small midgut gland tissue aliquots (~10 mg fresh weight) of at least three animals and processed for cDNA synthesis as detailed below.
The London School of Hygiene and Tropical Medicine looked into UK diets and found that healthier diets of fewer animal products and processed snacks, and more fruit and vegetables, can help reduce an astounding 40percentt of personal emissions.
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