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For each animal analyzed, a piece of frozen liver tissue was ground to fine powder in a mortar precooled in liquid nitrogen.
For each animal analyzed, the amount of fibrosis was measured as a percentage and the average value is presented [ 17].
From each animal analyzed, the amount of fibrosis was expressed as a percentage of the total liver area of 36 fields and the average value is presented.
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Representative images of animals analyzed are shown in Figure 3.
The animals analyzed belong to different families and were from different origins to avoid inbreeding effects.
This hypothesis must be qualified, however, given the small number of animals analyzed in both studies.
The total number of animals analyzed is listed in the figure legend.
This stereotyped red fluorescent pattern was consistently observed in all animals analyzed (n = 39).
Specifically, we observed hyperplasia of thymi, massive splenomegalies and lymphadenopathies in most of the animals analyzed (Figure 1B).
None of the animals analyzed possessed c.102del, indicating that c.102T>del is a relatively rare allele possessed by only one cynomolgus monkey (mfF1) (as a heterozygote) in this study.
IFN-γ was also increased though not significantly (Fig. 1D), and TNF-α was below detection limit in all animals analyzed (data not shown).
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