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All of the above experimental work including animal acquisition and preparation of venom protein fractions was performed by Professor Al-Saleh and coworkers [ 24, 27] and was provided to us as a gift.
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For self-administering animals, acquisition (day 1) was defined to be when the animal reached 35 or more responses, and animals normally reached acquisition criteria in 0 3 days.
In small laboratory animals, acquisition of detailed anatomical information, performance of dynamic scans or functional imaging with CT has specific challenges compared to imaging in humans.
Regardless of the exact evolutionary scenario of ICL, the unusual, for animals, acquisition of the bifunctional ICL-MS enzyme by nematodes via HGT from a bacterial source appears undeniable.
The location of the submerged platform was constant for each animal over acquisition trials, but varied across animals; starting position varied across trials.
An XYZ stage shifts the animal during acquisition for scanning large volumes up to the entire animal.
WZ: Participated in research design and animal experiment, acquisition of data and involved in drafting the manuscript.
Per each animal, the acquisition part starting at embedding was kept within 3 min to ensure fish health during time of measurement.
PO and HM were equally involved in conception and design of the study, surgical treatment and animal care, acquisition, analysis and interpretation of the data, and drafting of the manuscript and revising it critically for important intellectual content.
From previous literature [ 22], it is known that the signal to noise ratio (SNR) can influence this distribution, whether or not variances are caused by the animal or acquisition technique.
The role of animals in acquisition of NAP7 and NAP8/078/V strains was not evaluated because animal and food contact data were not available.
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