The results obtained in experiment 1.1 with the CUR loading geometry indicate that the contact angle, disk diameter, and "diameter-aggregates size-contact angle" interaction have a marginally significant contribution (0.050 < p ≤ 0.150).
A factorial design with four-factor and each factor with two levels were carried out to study the effects of the loading geometry, contact angle, disk diameter and thickness, aggregate size, and CS have on the measured ITS values.
In the first groups of experiments, the CS was held constant, and the effects of contact angle, disk diameter, aggregate size, and thickness of the disk on the standard deviation of the measured ITS values were studied.
The first group of experiments deals with the effects of the contact angle, disk diameter and thickness, and the maximum aggregate size on the variability of the measured ITS value.
Figure 4A shows the system bias between Gi and arrestin signaling for the D2R agonist quinpirole in three dose response projections (standard, rotated disk, and fixed-angle disk).
Pertussis toxin treatment of cells, by noncompetitively inhibiting Gi signaling, markedly drives the bias toward β-arrestin, and this is readily apparent in the fixed-angle disk model by a shift of points to the first quadrant.
In the third groups of experiments, with only one loading geometry, the maximum aggregate size was held constant, and the effects of the following factors on the measured ITS values were studied: contact angle, CS, disk diameter and thickness of the disk.
Data will be displayed in standard dose response curve format (projections of cylinder strings onto rectangular grids) and also as string projections onto rotated or fixed-angle Poincare disks oriented perpendicular to the cylindrical axis.
They video-taped the slowly falling disks, then digitized the images and had a computer measure the angle of the disk at intervals of 1/60 of a second.
Experiments were performed under different Reynolds number, 2800, 9000, and 36,000, and different values of inclination angle of the disk and jet-to-plate distance to jet diameter ratio H/Dh as 5, 10, and 15.
The exclusion criteria in the study eye were: intercurrent eye disease that is likely to affect VA over study period, presence of any diabetic retinopathy, other retinal vascular disease, vitreous hemorrhage, presence of neovascularization (iris, angle, retina and disk) or previous laser treatment.
