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Secondly, we deepened the anesthesia level with a supplemental intravenous anesthetic.
Animals were anesthetized by isoflurane inhalation in oxygen (the anesthesia level was maintaining by adjustment of the isoflurane concentration, 0.8 2.2 %).
A procedure's duration and anesthesia level would be addressed differently depending on the priority.
(Also, in a subset of animals, Arterial O2 saturation, respiration, and heart rate were measured using the MouseOx pulse oximetry unit (Starr Life Sciences, Allison Park, PA) to ensure that observed changes following pMCAO were not due to variability in vital parameters due to changes in anesthesia level).
It has a low blood/gas solubility coefficient that allows rapid changes in anesthesia level.
This method sets the blood concentration, and administration of anesthetic establishes a constant anesthesia level.
Similar(43)
CAPs categorized by K-means clustering were conserved at all anesthesia levels and wakefulness, although their proportion changed in a state-dependent manner.
Anesthesia levels were monitored by the recording of low-frequency electroencephalogram (EEG) and the absence of reflexes.
At surgical anesthesia levels (1.5 2%), isoflurane completely suppressed the electroencephalogram and silenced cortical neurons.
Vital signs were continuously monitored and kept constant by adjusting anesthesia levels.
However, because the PA and the LA displayed similar SO2 levels, potential confounding factors such as anesthesia levels could be excluded.
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